We studied the antitumor effect of recombinant human interferon alphα-2a (IFN α-2a) against human chronic myeloid leukemia cell line, KU-812F, which was transplanted in nude mice.
In nude mice the tumor inducing rate of KU-812E cell line (100%) which was selected as the cell line in this experiment was higher than that of K-562 cell line (20%). IFN α-2a treated group showed antitumor effect to max. 1×10^7IU without any toxicity. After the tumor weight reached 100-300㎣, the agents were administrated. In the group treated with IFN α-2a 1×10^6IU, disappearance of the tumor was observed and T/C (%) value was reduced to 35%. In the group treated with hydroxyurea 0.5㎎/g B.W., marked weight loss was observed. V_r/V_c value of the group was reduced but increased afterward. T/C(%) value of the group administrated with IFN α-2a 5×10^5IU and hydroxyurea 0.25㎎/g B.W. was reduced to 76% which was lower than that of the group treated with IFN α-2a 1×10^5IU. The tumor inducing rate was markedly reduced when the mice were inoculated IFN α-2a shortly after the tumor transplantation. In groups administrated with IFN α-2a 5×10^6IU and 1×10^7IU, 40% rejection rate in tumor transplantation was observed.
As a result, We confirmed that recombinant human IFN α-2a can inhibit the proliferation of chronic myelogenous leukemia cell(KU-812F) transplanted in nude mice.
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